spcas9 coding sequences Search Results


spcas9 coding sequences  (Addgene inc)
96
Addgene inc spcas9 coding sequences
The modified 3TC scaffold boosts <t>SpCas9</t> gRNA expression levels, compared to the original 4T scaffold. (A) DNA sequence of the 4T and modified 3TC scaffolds. (B) Relative quantification (RQ) of mDmd gRNA delivered by nucleofection of PX459.V2 (4T) to C2C12 cells, by qRT-PCR. Mean Log 2 RQ ± 95% CI; n=3. One-way ANOVA with Dunnett’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001. (C, D, E) Comparison of the relative quantities of mDmd Sp gRNA, delivered by PX459.V2, pdg459.V2 (2x4T) and PX459.V3 (3TC), measured by qRT-PCR. Mean Log 2 RQ ± 95% CI; n=3. One-way ANOVA with Tukey’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001.
Spcas9 Coding Sequences, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ultra one step cloning kit v2  (Vazyme Biotech Co)
96
Vazyme Biotech Co ultra one step cloning kit v2
The modified 3TC scaffold boosts <t>SpCas9</t> gRNA expression levels, compared to the original 4T scaffold. (A) DNA sequence of the 4T and modified 3TC scaffolds. (B) Relative quantification (RQ) of mDmd gRNA delivered by nucleofection of PX459.V2 (4T) to C2C12 cells, by qRT-PCR. Mean Log 2 RQ ± 95% CI; n=3. One-way ANOVA with Dunnett’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001. (C, D, E) Comparison of the relative quantities of mDmd Sp gRNA, delivered by PX459.V2, pdg459.V2 (2x4T) and PX459.V3 (3TC), measured by qRT-PCR. Mean Log 2 RQ ± 95% CI; n=3. One-way ANOVA with Tukey’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001.
Ultra One Step Cloning Kit V2, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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px330 ng  (Addgene inc)
93
Addgene inc px330 ng
The modified 3TC scaffold boosts <t>SpCas9</t> gRNA expression levels, compared to the original 4T scaffold. (A) DNA sequence of the 4T and modified 3TC scaffolds. (B) Relative quantification (RQ) of mDmd gRNA delivered by nucleofection of PX459.V2 (4T) to C2C12 cells, by qRT-PCR. Mean Log 2 RQ ± 95% CI; n=3. One-way ANOVA with Dunnett’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001. (C, D, E) Comparison of the relative quantities of mDmd Sp gRNA, delivered by PX459.V2, pdg459.V2 (2x4T) and PX459.V3 (3TC), measured by qRT-PCR. Mean Log 2 RQ ± 95% CI; n=3. One-way ANOVA with Tukey’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001.
Px330 Ng, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid constructions plasmids px330  (Addgene inc)
93
Addgene inc plasmid constructions plasmids px330
The modified 3TC scaffold boosts <t>SpCas9</t> gRNA expression levels, compared to the original 4T scaffold. (A) DNA sequence of the 4T and modified 3TC scaffolds. (B) Relative quantification (RQ) of mDmd gRNA delivered by nucleofection of PX459.V2 (4T) to C2C12 cells, by qRT-PCR. Mean Log 2 RQ ± 95% CI; n=3. One-way ANOVA with Dunnett’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001. (C, D, E) Comparison of the relative quantities of mDmd Sp gRNA, delivered by PX459.V2, pdg459.V2 (2x4T) and PX459.V3 (3TC), measured by qRT-PCR. Mean Log 2 RQ ± 95% CI; n=3. One-way ANOVA with Tukey’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001.
Plasmid Constructions Plasmids Px330, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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spcas9 hf1 coding sequence  (Addgene inc)
93
Addgene inc spcas9 hf1 coding sequence
The modified 3TC scaffold boosts <t>SpCas9</t> gRNA expression levels, compared to the original 4T scaffold. (A) DNA sequence of the 4T and modified 3TC scaffolds. (B) Relative quantification (RQ) of mDmd gRNA delivered by nucleofection of PX459.V2 (4T) to C2C12 cells, by qRT-PCR. Mean Log 2 RQ ± 95% CI; n=3. One-way ANOVA with Dunnett’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001. (C, D, E) Comparison of the relative quantities of mDmd Sp gRNA, delivered by PX459.V2, pdg459.V2 (2x4T) and PX459.V3 (3TC), measured by qRT-PCR. Mean Log 2 RQ ± 95% CI; n=3. One-way ANOVA with Tukey’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001.
Spcas9 Hf1 Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ppbu6 hdhfr yfcu cas9  (Addgene inc)
93
Addgene inc ppbu6 hdhfr yfcu cas9
The modified 3TC scaffold boosts <t>SpCas9</t> gRNA expression levels, compared to the original 4T scaffold. (A) DNA sequence of the 4T and modified 3TC scaffolds. (B) Relative quantification (RQ) of mDmd gRNA delivered by nucleofection of PX459.V2 (4T) to C2C12 cells, by qRT-PCR. Mean Log 2 RQ ± 95% CI; n=3. One-way ANOVA with Dunnett’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001. (C, D, E) Comparison of the relative quantities of mDmd Sp gRNA, delivered by PX459.V2, pdg459.V2 (2x4T) and PX459.V3 (3TC), measured by qRT-PCR. Mean Log 2 RQ ± 95% CI; n=3. One-way ANOVA with Tukey’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001.
Ppbu6 Hdhfr Yfcu Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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spcas9 ng  (Addgene inc)
93
Addgene inc spcas9 ng
The modified 3TC scaffold boosts <t>SpCas9</t> gRNA expression levels, compared to the original 4T scaffold. (A) DNA sequence of the 4T and modified 3TC scaffolds. (B) Relative quantification (RQ) of mDmd gRNA delivered by nucleofection of PX459.V2 (4T) to C2C12 cells, by qRT-PCR. Mean Log 2 RQ ± 95% CI; n=3. One-way ANOVA with Dunnett’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001. (C, D, E) Comparison of the relative quantities of mDmd Sp gRNA, delivered by PX459.V2, pdg459.V2 (2x4T) and PX459.V3 (3TC), measured by qRT-PCR. Mean Log 2 RQ ± 95% CI; n=3. One-way ANOVA with Tukey’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001.
Spcas9 Ng, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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spcas9  (Addgene inc)
95
Addgene inc spcas9
The modified 3TC scaffold boosts <t>SpCas9</t> gRNA expression levels, compared to the original 4T scaffold. (A) DNA sequence of the 4T and modified 3TC scaffolds. (B) Relative quantification (RQ) of mDmd gRNA delivered by nucleofection of PX459.V2 (4T) to C2C12 cells, by qRT-PCR. Mean Log 2 RQ ± 95% CI; n=3. One-way ANOVA with Dunnett’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001. (C, D, E) Comparison of the relative quantities of mDmd Sp gRNA, delivered by PX459.V2, pdg459.V2 (2x4T) and PX459.V3 (3TC), measured by qRT-PCR. Mean Log 2 RQ ± 95% CI; n=3. One-way ANOVA with Tukey’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001.
Spcas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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coding sequence for spcas9  (Addgene inc)
96
Addgene inc coding sequence for spcas9
The modified 3TC scaffold boosts <t>SpCas9</t> gRNA expression levels, compared to the original 4T scaffold. (A) DNA sequence of the 4T and modified 3TC scaffolds. (B) Relative quantification (RQ) of mDmd gRNA delivered by nucleofection of PX459.V2 (4T) to C2C12 cells, by qRT-PCR. Mean Log 2 RQ ± 95% CI; n=3. One-way ANOVA with Dunnett’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001. (C, D, E) Comparison of the relative quantities of mDmd Sp gRNA, delivered by PX459.V2, pdg459.V2 (2x4T) and PX459.V3 (3TC), measured by qRT-PCR. Mean Log 2 RQ ± 95% CI; n=3. One-way ANOVA with Tukey’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001.
Coding Sequence For Spcas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
coding sequence for spcas9 - by Bioz Stars, 2026-08
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Image Search Results


The modified 3TC scaffold boosts SpCas9 gRNA expression levels, compared to the original 4T scaffold. (A) DNA sequence of the 4T and modified 3TC scaffolds. (B) Relative quantification (RQ) of mDmd gRNA delivered by nucleofection of PX459.V2 (4T) to C2C12 cells, by qRT-PCR. Mean Log 2 RQ ± 95% CI; n=3. One-way ANOVA with Dunnett’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001. (C, D, E) Comparison of the relative quantities of mDmd Sp gRNA, delivered by PX459.V2, pdg459.V2 (2x4T) and PX459.V3 (3TC), measured by qRT-PCR. Mean Log 2 RQ ± 95% CI; n=3. One-way ANOVA with Tukey’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001.

Journal: bioRxiv

Article Title: Enhancing gRNA Transcript levels by Reducing the Scaffold Poly-T Tract for Optimal SpCas9- and SaCas9-mediated Gene Editing

doi: 10.1101/2024.07.19.604224

Figure Lengend Snippet: The modified 3TC scaffold boosts SpCas9 gRNA expression levels, compared to the original 4T scaffold. (A) DNA sequence of the 4T and modified 3TC scaffolds. (B) Relative quantification (RQ) of mDmd gRNA delivered by nucleofection of PX459.V2 (4T) to C2C12 cells, by qRT-PCR. Mean Log 2 RQ ± 95% CI; n=3. One-way ANOVA with Dunnett’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001. (C, D, E) Comparison of the relative quantities of mDmd Sp gRNA, delivered by PX459.V2, pdg459.V2 (2x4T) and PX459.V3 (3TC), measured by qRT-PCR. Mean Log 2 RQ ± 95% CI; n=3. One-way ANOVA with Tukey’s multiple comparisons test performed on ΔΔCT values, **p ≤ 0.01, ***p ≤ 0.001.

Article Snippet: SaCas9-Puro.V2 was generated by replacing the SpCas9 coding sequences of PX459.V2 with SaCas9 coding sequences from PX601 (Addgene #61591).

Techniques: Modification, Expressing, Sequencing, Quantitative Proteomics, Quantitative RT-PCR, Comparison

Editing efficiencies of high-fidelity SpCas9s with the 3TC scaffold. Comparison of PX459.V2 SpCas9-HF1 (4T), PX459.V3 SpCas9-HF1 (3TC), PX459.V2 eSpCas9(1.1) (4T) and PX459.V3 eSpCas9(1.1) (3TC) plasmids delivered by lipofection at a (A) high and (B) low plasmid dose without puromycin selection in HEK239T cells, assessed by deep amplicon sequencing. Mean ± SEM; n=3. Two-way ANOVA with Šídák’s multiple comparisons test; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. (C) Editing efficiencies of hDMD-B in the G19 gRNA configuration with WT and high-fidelity Sp-Cas9 plasmids delivered by nucleofection with puromycin selection in HEK293Ts. Two-way ANOVA with Šídák’s multiple comparisons test; *p ≤ 0.05, ***p ≤ 0.001.

Journal: bioRxiv

Article Title: Enhancing gRNA Transcript levels by Reducing the Scaffold Poly-T Tract for Optimal SpCas9- and SaCas9-mediated Gene Editing

doi: 10.1101/2024.07.19.604224

Figure Lengend Snippet: Editing efficiencies of high-fidelity SpCas9s with the 3TC scaffold. Comparison of PX459.V2 SpCas9-HF1 (4T), PX459.V3 SpCas9-HF1 (3TC), PX459.V2 eSpCas9(1.1) (4T) and PX459.V3 eSpCas9(1.1) (3TC) plasmids delivered by lipofection at a (A) high and (B) low plasmid dose without puromycin selection in HEK239T cells, assessed by deep amplicon sequencing. Mean ± SEM; n=3. Two-way ANOVA with Šídák’s multiple comparisons test; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. (C) Editing efficiencies of hDMD-B in the G19 gRNA configuration with WT and high-fidelity Sp-Cas9 plasmids delivered by nucleofection with puromycin selection in HEK293Ts. Two-way ANOVA with Šídák’s multiple comparisons test; *p ≤ 0.05, ***p ≤ 0.001.

Article Snippet: SaCas9-Puro.V2 was generated by replacing the SpCas9 coding sequences of PX459.V2 with SaCas9 coding sequences from PX601 (Addgene #61591).

Techniques: Comparison, Plasmid Preparation, Selection, Amplification, Sequencing